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[Quantitative approach of melanin in human cultured melanocytes].
M Boue-Grabot, Yvon Gauthier, Jean Cambar
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Abstract
The purpose of the study was to establish an in vitro model for the quantification of the melanin produced in human melanocytes in culture. Melanin content in these cultured human melanocytes is determined by spectrofluorimetric assay. Fluorescence intensity is quantitatively related to the melanin content present in cultured melanocytes.