Immunoblot analysis ofDirofilaria immitisrecognized by infected humans

To help to develop better diagnostic tests for zoonotic pulmonary dirofilariasis, seven patients with histologically confirmed pulmonary dirofilariasis were evaluated using the immunoblot technique. Six patients exhibited a response to the excretory-secretory (AS) antigen proteins with molecular weights of 20-19.5, 17.5-17 and 14 derived from Dirofilaria immitis adult works. However, when adult worm extracts were used as the antigen, there was no qualitative difference in antigen recognition between the Dirofilaria patients, other patients with non-filarial parasitic infections or with lung cancer or tuberculosis, and normal individuals. All the sera, not only those of the Dirofilaria patients but also those of the non-filarial patients and the controls, strongly cross-reacted with the 18-kDa ES antigen. These findings suggest that the ES antigen of D. immitis provides a more sensitive antigen than does the adult somatic antigen; but the ES antigen and the adult somatic antigen have a common antigenic band at 18 kDa.