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[Meiotic maturation of the ovocyte of Xenopus laevis : a 4-step mechanism].

Denise Huchon, René Ozon, E H Fischer, Demaille Jg

Abstract

Microinjection of the pure inhibitor (1) of cAMP dependent protein kinases (1.5 muM) into Xenopus oocytes triggered 100% germinal vesicle break down (GVBD), faster than progesterone and slower than the maturation promoting factor (MPF). Cholera toxin does not block (I) induced maturation; however cycloheximide inhibits (I) but not MPF induced GVBD.

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Available abstract

Key concepts: Xenopus, Germinal vesicle, Microinjection, Cycloheximide, Cell biology, Maturation promoting factor, Cholera toxin, Biology