2002•PubMedRequires access
[Cloning differentially expressed genes by suppression subtractive hybridization in rat liver regeneration].
Yuchang Li, Cunshuan Xu, Yunhan Zhang
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Abstract
The cDNA from rat regenerating liver tissue was used as the tester and that from normal liver was used as the driver. A highly efficient subtractive cDNA library was constructed by suppression subtractive hybridization(SSH). After screening, 31 clones from 50 clones which were derived from the cDNA library were inserted by 60-400 bp cDNA fragments. 24 cDNA fragments corresponded to known genes and 7 cDNA fragments were unknown sequences (GenBank accession number: BG447490-447496).