Histochemical and biochemical study on the acetylcholinesterase and choline acetyltransferase in the brain and spinal cord of frog, Rana esculenta.

The histochemical distribution of acetylcholinesterase (AChE) activity in the brain and spinal cord of Rana esculenta, has been studied. Moreover the levels of choline acetyl-transferase (ChAT) and of AChE, have been determined biochemically in different regions of the central nervous system. The results obtained from combining the histochemical and biochemical analyses allowed us to obtain more significant information on the localization of cholinergic neurons and the projection areas of the cholinergic terminals.