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[The determination of the site specificity of type-II restriction endonuclease in Streptomyces sp. 48].

Luk'ianchuk Vv, Л. В. Поліщук, Stryzhkova Hm, O P Kopieĭko, Matseliukh Bp

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Abstract

The activity of restriction endonuclease which splits DNA of lambda and T7 phages into 8 fragments has been detected in the mycelium lysates of the 2-days culture of Streptomyces sp. 48. The fragments dimensions according to the data of agarose gel electrophoresis, coincide with those of AsuII--fragments of DNA of lambda and T7 phages. This allows the studied Ssp48 restrictase to be considered the isoschizomer of AsuII restriction endonuclease.

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What this paper is about

The activity of restriction endonuclease which splits DNA of lambda and T7 phages into 8 fragments has been detected in the mycelium lysates of the 2-days culture of Streptomyces sp. 48. The fragments dimensions according to the data of agarose gel electrophoresis, coincide with those of AsuII--fragments of DNA of lambda and T7 phages. This allows the studied Ssp48 restrictase to be considered the isoschizomer of AsuII restriction endonuclease.

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Available abstract

The activity of restriction endonuclease which splits DNA of lambda and T7 phages into 8 fragments has been detected in the mycelium lysates of the 2-days culture of Streptomyces sp. 48. The fragments dimensions according to the data of agarose gel electrophoresis, coincide with those of AsuII--fragments of DNA of lambda and T7 phages. This allows the studied Ssp48 restrictase to be considered the isoschizomer of AsuII restriction endonuclease.

Key concepts: Isoschizomer, Restriction enzyme, Endonuclease, Molecular biology, Biology, Agarose, Agarose gel electrophoresis, Streptomyces

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