2009•PubMedRequires access

[Study on the extraction technology of polysaccharides from Caulis Marsdeniae Tenacissimae].

Bin Li, Xin-yun Liu, Hui Zhang, Ting‐Guo Kang

Abstract

OBJECTIVE: To optimize the extraction technology and to establish the determination method of polysaccharides from Caulis Marsdeniae Tenacissimae. METHODS: Phenol-sulphate colorimetry was used to determine the content of polysaccharides. According to the yield of polysaccharides, the orthogonal design was used to select the extraction technology of polysaccharides. RESULTS: The optimum extraction procedure was 8 folds of water, refluxing and extracting for 3 times at 100 degrees C, and 1 h for each time. The concentration of polysaccharide was linear in the range of 0.004-0.014 mg/mL The average recovery was 99.72%, RSD = 0. 63%. CONCLUSION: The optimum extraction procedure is simple and reliable which is suitable for industrial production. The method for determination is simple, specific and accurate which can provide a method for mass control of Marsdenia tenacissima.

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Key concepts: Polysaccharide, Extraction (chemistry), Chromatography, Colorimetry, Chemistry, Yield (engineering), Materials science, Biochemistry