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[Method of measurement of protein metabolism in isolated skeletal muscle of the rat].

Roman Šafránek, Milan J. Holecek, Jana Kadlcíková, Jaromír Chládek, Csilla Mišľanová, Marián Kukan

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Abstract

The aim of our present research is to evaluate the direct effects of humoral factors on protein metabolism. For this purpose we have adopted a method that enables measuring of parameters of protein and amino acid metabolism in isolated m. soleus and m. extensor digitorum longus of the rat. The rate of tyrosine release was used to estimate protein breakdown. The oxidation of leucine was evaluated by the rate of 14CO2 production from [1-14C]leucine. Incorporation of [1-14C]leucine into muscle proteins was used to estimate protein synthesis. The metabolic state of the muscles was evaluated by measuring changes in ATP, ADP, AMP levels, and calculated energy charge.

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What this paper is about

The aim of our present research is to evaluate the direct effects of humoral factors on protein metabolism. For this purpose we have adopted a method that enables measuring of parameters of protein and amino acid metabolism in isolated m. soleus and m. extensor digitorum longus of the rat. The rate of tyrosine release was used to estimate protein breakdown. The oxidation of leucine was evaluated by the rate of 14CO2 production from [1-14C]leucine. Incorporation of [1-14C]leucine into muscle proteins was used to estimate protein synthesis. The metabolic state of the muscles was evaluated by measuring changes in ATP, ADP, AMP levels, and calculated energy charge.

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Available abstract

The aim of our present research is to evaluate the direct effects of humoral factors on protein metabolism. For this purpose we have adopted a method that enables measuring of parameters of protein and amino acid metabolism in isolated m. soleus and m. extensor digitorum longus of the rat. The rate of tyrosine release was used to estimate protein breakdown. The oxidation of leucine was evaluated by the rate of 14CO2 production from [1-14C]leucine. Incorporation of [1-14C]leucine into muscle proteins was used to estimate protein synthesis. The metabolic state of the muscles was evaluated by measuring changes in ATP, ADP, AMP levels, and calculated energy charge.

Key concepts: Leucine, Protein metabolism, Tyrosine, Metabolism, Muscle protein, Amino acid, Biochemistry, Energy metabolism

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[Method of measurement of protein metabolism in isolated skeletal muscle of the rat]. — Research Paper | ScholarLens