[Effects of different expression levels of fibronectin on biological behavior of tumor cells].

OBJECTIVE: To study the effects of fibronectin(FN) on tumor cell proliferation, adherence and invasion. METHODS: FN cDNA was introduced into a transitional carcinoma cell line 253J which expressed low level of FN, and 253 FN line which expressed higher level of FN was established. The changes of cellular proliferation in vitro were observed. Adhesiveness to the extracellular matrix was detected by MTT method. Hemotypic adhesion was investigated by detachment assay and invasiveness of the cells was observed with Boyden Chamber before and after FN cDNA gene transfection. RESULTS: The growth rate of 253-FN was decreased as compared with its parental cell 253J. Hemotypic adhesion was significantly increased. The adhesiveness of the cells to extracellular matrix was enhanced. Invasion assay showed that invasioness of 253-FN was decreased. CONCLUSION: This study suggested a correlation between FN expression and its biological behavior. With increased FN level, malignant phenotype was changed and invasive ability was inhibited.