Multiplication of Pseudomonas aeruginosa in vitro

In the previous paper, it was demonstrated that Pseudomonas aeruginosa propagated in city water, but did not grow when the city water was deionized by an ion-exchange resin. In order to know what kind of component in city water is essential for the growth of the bacilli, some fundamental experiments were performed.The present paper primarily describes the studies on the growth of Pseudomonas aeruginosa in simple solutions artificially composed of inorganic salts only.The results obtained are as follows:1) It was noted that Pseudomonas aeruginosa was able to grow in inorganic salt solutions. It was clearly determined that, within the limited studies performed here, the bacilli easily multiplied in the solution containing only CaCl2 and Na2HPO4 at the final concentration of 0.01%.2) It was further confirmed that Pseudomonas aeruginosa definitely multiplied in the mixture solution of CaCl2 and Na2HPO4, even when the inoculum size was extraordinarly small.3) Furthermore, the serial subcultivation of the bacilli in this mixture solution was possible. Therefore, it could be mentioned that the phenomenon that the bacilli multiplied in this solution should not be false but genuine growth.The data, repo' ted here, concerning the discovery of the minimum essential growth factor for Pseudomonas aeruginosashould contribute to the studies on drug-fastness of this organism and reconstruction of bacterial cells.