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Histocompatibility testing in dogs. I. A semi-micro mixed lymphocyte culture (MLC) technique for histocompatibility matching in dogs.

Goldmann Sf, Flad Hd

Abstract

A semi-micro method for the mixed lymphocyte culture (MLC) test in dogs is described. Optimization of various factors influencing the test were invistigated. Discrimination between allogeneic and isogeneic cell mixtures was possible after 90 h or culture but a culture period of 144 h was found to be optimal. The method measures MLC identity or non-identity and can be used as a tool for histocompatibility testing of lymphocyte defined (LD) Antigens.

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Key concepts: Histocompatibility, Histocompatibility Testing, Immunology, Antigen, Lymphocyte, Biology, Major histocompatibility complex, Human leukocyte antigen