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[Analysis of saponins from panax notoginseng using pressurized solvent extraction coupled with liquid chromatography-electrospray mass spectrum].

Jian‐Bo Wan, Chang Ming Li, Shaopin Li, Ling‐Yi Kong, Yitao Wang

Abstract

OBJECTIVE: To establish a method for qualitative analysis of saponins from Panax notoginseng using pressurized solvent extraction coupled with LC-ESI-MS. METHODS: The PSE technology was applied to the process of extraction for Panax notoginseng, and the negative ion detection and multiple reaction monitoring model were used. The saponins were investigated based on total ion chromatogram (TIC) and MRM chromatogram. RESULTS: According to the fragment character of saponins, the molecular weight and their structures could be identified. CONCLUSION: The method can be used for qualitative analysis of saponins from Panax notoginseng.

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Available abstract

Key concepts: Panax notoginseng, Chromatography, Chemistry, Extraction (chemistry), Saponin, Accelerated solvent extraction, Solvent, Electrospray