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[Cloning of the tag7 gene expressed in metastatic mouse tumors].

Olga S. Kustikova, С. Л. Киселев, Olga R. Borodulina, V. M. Senin, A V Afanas'eva, Andrei A. Kabishev

Abstract

Gene expression was compared in a metastatic (VMR-Liv) neoplastic cell line and a related nonmetastatic (VMR-O) neoplastic cell line by means of the differential display method. A fragment of cDNA corresponding to the tag7 gene, differentially expressed in the metastatic cell line, was isolated. The full-length tag7 cDNA was cloned and its nucleotide sequence was determined. No homology between the tag7 gene and known sequences was revealed. tag7 gene transcription was studied in some tumors, cell lines, and normal mouse organs.

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Key concepts: Complementary DNA, Gene, Molecular biology, Cloning (programming), Biology, Homology (biology), Gene expression, Differential display