2005•PubMedRequires access
[Expression and antigenicity analysis of p46000 antigen from newborn larvae of Trichinella spiralis].
Lihong Yuan, Bao‐Quan Fu, Mingyuan Liu, Fei Gao, Yalan Zhang, Xiuping Wu, Ben-fu Lin, Lianrui Li, Qiang Lu, Qijun Chen, Pascal Boireau
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Abstract
OBJECTIVE: To express and analyze p46000 antigen from newborn larvae of Trichinella spiralis. METHODS: p46000 antigen gene was subcloned to the pET28a expression system by PCR. The recombinant transformant was induced by IPTG and the antigenicity was analyzed with ELISA and Western blotting. RESULTS: The molecular weight of the expressed protein was about Mr 48000. ELISA and Western blotting showed that this recombinant protein could be recognized by T. spiralis infected swine serum and rabbit anti-recombinant protein serum, and the rabbit anti-recombinant protein serum could recognize a Mr 46000 protein from newborn larvae of T. spiralis. CONCLUSION: p46000 recombinant antigen from newborn larvae of T. spiralis was expressed which shows a specific antigenicity.