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Evaluation of IgM-ELISA versus PCR in diagnosis of recent Toxoplasma gondii infection.

Atef Hussein, Ibraheim M Nagaty, Mahmoud A H Fouad

Open publisher page 6 citations

Abstract

Whereas Toxoplasma gondii infection in the immunocompetent adult usually causes no serious clinical symptoms, congenital infection can lead to abortion or severe disease in the newborn infant. Early diagnosis should be made as soon as possible particularly in pregnant women and newborn babies since early treatment can minimize fetal sequelae. This work evaluates IgM-ELISA and PCR in diagnosis of recent T. gondii infection. The results revealed that PCR detected very recently infected cases (23 out of 70 suspected cases) than IgM-ELISA (only 18 cases). The combination of both tests may help to improve the sensitivity of diagnosis (45% in suspected group) more than either PCR (32.8%) or IgM-ELISA alone (25.7%).

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What this paper is about

Whereas Toxoplasma gondii infection in the immunocompetent adult usually causes no serious clinical symptoms, congenital infection can lead to abortion or severe disease in the newborn infant. Early diagnosis should be made as soon as possible particularly in pregnant women and newborn babies since early treatment can minimize fetal sequelae. This work evaluates IgM-ELISA and PCR in diagnosis of recent T. gondii infection. The results revealed that PCR detected very recently infected cases (23 out of 70 suspected cases) than IgM-ELISA (only 18 cases). The combination of both tests may help to improve the sensitivity of diagnosis (45% in suspected group) more than either PCR (32.8%) or IgM-ELISA alone (25.7%).

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OpenAlex reports 6 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Whereas Toxoplasma gondii infection in the immunocompetent adult usually causes no serious clinical symptoms, congenital infection can lead to abortion or severe disease in the newborn infant. Early diagnosis should be made as soon as possible particularly in pregnant women and newborn babies since early treatment can minimize fetal sequelae. This work evaluates IgM-ELISA and PCR in diagnosis of recent T. gondii infection. The results revealed that PCR detected very recently infected cases (23 out of 70 suspected cases) than IgM-ELISA (only 18 cases). The combination of both tests may help to improve the sensitivity of diagnosis (45% in suspected group) more than either PCR (32.8%) or IgM-ELISA alone (25.7%).

Key concepts: Toxoplasma gondii, Medicine, Toxoplasmosis, Immunology, Immunoglobulin M, Abortion, Clinical diagnosis, Pregnancy

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Evaluation of IgM-ELISA versus PCR in diagnosis of recent Toxoplasma gondii infection. — Research Paper | ScholarLens