Purification of an anti-lipoprotein lipase antiserum.

Lipoprotein lipase is an enzyme difficult to isolate in pure form and, until now, the antisera prepared against it have not been monospecific. The present experiments show how a crude antiserum prepared with bovine milk lipoprotein lipase, can be made more specific through suitable adsorption. The crude antiserum was prepared by injecting milk lipoprotein lipase prepared by heparin Sepharose affinity chromatography. Immunodiffusion techniques indicated that the antiserum contained antibodies to proteins other than lipoprotein lipase (bovine milk and serum proteins) and that these antibodies could be eliminated by adsorption with bovine serum and a beta-casein preparation.