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[Serological characteristics of KAMA-51 monoclonal antibodies to the tick-borne encephalitis virus].
S Ia Gaĭdamovich, E E Mel'nikova, A S Novokhatskiĭ, Kushch Aa, N A Sveshnikova
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Abstract
KAMA-51 monoclonal antibodies to tick-borne encephalitis virus are produced by hybridoma obtained by fusion of splenocytes of mice immunized with this virus with myeloma X-653 cells. The antibody belongs to the IgG class, is active in the immunofluorescence test (IFT), does not react in CFT, and has no antihemagglutinating or neutralizing properties. In the IFT, it reacts with all viruses of the tick-borne encephalitis complex indicating their directivity to the groupspecific determinant of E protein. In the indirect IFT, antibody titres in the culture fluid are within the range of 1: 16-1: 62, in the ascitic fluids 1: 320-1: 640. Because of the wide range of interspecies reactions, KAMA-51 monoclonal antibody may be used for group detection of the tick-borne encephalitis complex viruses.