ScholarLens Back to search
2005•PubMedRequires access

[Effect of Herba Epimedii Flavone on expression of OPG and RANKL in rat osteoblasts].

Yiheng Liu, Hongmin Zang, Haiying Zhang, Junchang Chen

Open publisher page 7 citations

Abstract

OBJECTIVE: To explore the effect of Herba Epimedii Flavone (HEF) on expression of OPG and RANKL in rat osteoblasts. METHODS: Osteoblasts were obtained from new born rat calvaria by digestive enzymes. The different HEF concentrations (0.1, 1, 10, 100 ng/ml)were added into the culture medium. Total mRNA was prepared from osteoblasts after 48 h and 96 h, and mRNA expression of OPG and RANKL were detected by RT-PCR. RESULTS: The mRNA expression of OPG in osteoblasts significantly increased compared with that of the control group, and the V(OPF)/V(RANKL) ratio was deceased markedly in the treatment of 10 ng/ml group after 48 h. After 96 h, the mRNA expression of OPG significantly increased and the V(OPG)/V(RANKL) ratio was also decreased in the treatment group compared with that of the control group. CONCLUSION: HEF suppress the differentiation and maturate of osteoblast. Osteoporosis can be prevented and cured by HEF.

About this research paper

What this paper is about

OBJECTIVE: To explore the effect of Herba Epimedii Flavone (HEF) on expression of OPG and RANKL in rat osteoblasts. METHODS: Osteoblasts were obtained from new born rat calvaria by digestive enzymes. The different HEF concentrations (0.1, 1, 10, 100 ng/ml)were added into the culture medium. Total mRNA was prepared from osteoblasts after 48 h and 96 h, and mRNA expression of OPG and RANKL were detected by RT-PCR. RESULTS: The mRNA expression of OPG in osteoblasts significantly increased compared with that of the control group, and the V(OPF)/V(RANKL) ratio was deceased markedly in the treatment of 10 ng/ml group after 48 h. After 96 h, the mRNA expression of OPG significantly increased and the V(OPG)/V(RANKL) ratio was also decreased in the treatment group compared with that of the control group. CONCLUSION: HEF suppress the differentiation and maturate of osteoblast. Osteoporosis can be prevented and cured by HEF.

Why it matters

OpenAlex reports 7 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE: To explore the effect of Herba Epimedii Flavone (HEF) on expression of OPG and RANKL in rat osteoblasts. METHODS: Osteoblasts were obtained from new born rat calvaria by digestive enzymes. The different HEF concentrations (0.1, 1, 10, 100 ng/ml)were added into the culture medium. Total mRNA was prepared from osteoblasts after 48 h and 96 h, and mRNA expression of OPG and RANKL were detected by RT-PCR. RESULTS: The mRNA expression of OPG in osteoblasts significantly increased compared with that of the control group, and the V(OPF)/V(RANKL) ratio was deceased markedly in the treatment of 10 ng/ml group after 48 h. After 96 h, the mRNA expression of OPG significantly increased and the V(OPG)/V(RANKL) ratio was also decreased in the treatment group compared with that of the control group. CONCLUSION: HEF suppress the differentiation and maturate of osteoblast. Osteoporosis can be prevented and cured by HEF.

Key concepts: RANKL, Calvaria, Chemistry, Endocrinology, Osteoblast, Osteoprotegerin, Internal medicine, Messenger RNA

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
[Effect of Herba Epimedii Flavone on expression of OPG and RANKL in rat osteoblasts]. — Research Paper | ScholarLens