The effect of the rat-cyclophosphamide serum on the rat hematopoietic tissue recovery, previously damaged by cyclophosphamide or by gamma-irradiation.

The effect of post-cyclophosphamide serum (post-CY serum) humoral activity in vivo, on the rat hematopoietic regeneration after damage either by cyclophosphamide (CY) or by gamma-irradiation was investigated. Three experimental groups of rats were studied. The first group was treated by a single high dose of CY (200 mg/kg), the second group was whole body irradiated by 6.5 Gy, and the third group was whole body irradiated by 3.5 Gy. Each group was subdivided into three subgroups. The first among subgroups received immediately after damage 0.5 cc post-CY serum, the second received 0.5 normal rat sera per animal. The third subgroup of rats was used as control. Post-CY sera were collected from the Wistar rats, 2.5 months old 48 hours after the treatment by high single dose by CY (300 mg/kg). Post-CY serum activity in comparison to normal serum activity was evaluated in vivo conditions. In order to take samples of peripheral blood, spleen and bone marrow, the animals were sacrificed 24 hours, 48 hours, 72 hours and seven days after treatment. The assessment of post-CY sera effect is accomplished by measuring of the proliferative capacity of splenic, as well as bone marrow cells, and by means of the RBC and WBC counts. The results obtained indicate substantial stimulative and proliferative activity of the post-CY sera, when compared to normal sera, particularly in the animals previously damaged with CY.