The relationship between hydrogenase and nitrogenase for hydrogen evolution in Rhodopseudomonas sp. KCTC 1437

Both hydrogenase and nitrogenase were found to be involved in hydrogen evolution independently in Rhodopseudomonas sp. KCTC 1437. The hydrogen formation in this bacterium was independent on light illumination and presence of N After establishment of conditions to measure the amount of hydrogen evolved by each of the enzymes in vivo, the several factors affecting on the hydrogen evolution, e.g. presence of gases ( , , or ), C/N ratio, were investigated, Hydrogenase was less inhibited than nitrogenase under and was active independent on the presence of or which were the strong inhibitor of nitrogenase. Besides, the hydrogenase activity was increased after incubation with . And it was verified that this bacterium consume hydrogen and photoreduce by hydrogenase. From above results, it is concluded that hydrogenase in Rhodopseudomonas sp. KCTC 1437 can produce hydrogen under more favorable condition that nitrogenase.e.