2003•PubMedRequires access
[Expressions of TNF-alpha and IL-1beta in human ischemic brain tissues].
Zhaohua Zhong, Guozhong Li, Hulun Li, Wen-Ran Zhao, Ye Tian, Dianjun Li, Hong-Xi Gu, Haitao Wang, Dong Xiu-qin
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Abstract
AIM: To detect the expression of TNF-alpha and IL-1beta in human cerebral ischemic tissues. METHODS: 13 cerebral specimens from patients died of cerebral infarction were divided into three groups, <2 d, 3-5 d, and >5d, according to the lasting time of infarctions. The expression of TNF-alpha and IL-1beta in the cerebral ischemic tissues were examined by immnohistochemical staining. The contralateral tissues were employed as controls. RESULTS: The expression of TNF-alpha and IL-1beta in the cerebral ischemic tissues were significant higher than those in the contralateral tissues. The focal distribution of the TNF-alpha(+) and IL-1beta(+) cells was identical with the ischemic area. The expression of IL-1beta and TNF-alpha peaked at the 3rd to 5th and 2nd day after ichemia, respectively. There were no significant difference between the ischemic and contralateral brains at the 5th day after ischemia for the expression of TNF-alpha and IL-1beta. CONCLUSION: Our results showed the expression of TNF-alpha and IL-1beta in human strok of infarction were similar to those in animal experiments. It is suggested that TNF-alpha and IL-1beta are involved in cerebral ischemic injury, which will be helpful for developing clinically a novel therapy aiming at cerebral ischemic injury.