1994•PubMedRequires access
[The use of pyrophosphate-dependent phosphofructokinase for the determination of fructose-2,6-bisphosphate concentration in biological material].
Golubev Ma, Markova Ms
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Abstract
The authors discuss methods for purification of pyrophosphate fructose-6-phosphate phosphotransferase from potato tuber and enzymatic method for measuring fructose-2,6-biphosphate concentration in isolated hepatocytes of normal and diabetic rats and come to a conclusion on a higher sensitivity of the method they propose in comparison with the method making use of commercial phosphofructokinase from rabbit muscles. Their method may be used for fructose-2,6-biphosphate measurements in various biologic materials.