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Selective media for Pasteurella multocida and Bordetella bronchiseptica.

L E Garlinghouse, DiGiacomo Rf, Van Hoosier Gl, J Condon

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Abstract

Easily prepared and inexpensive culture medium for the isolation of Pasteurella multocida and Bordetella bronchiseptica from the nares of laboratory rabbits was prepared by incorporating 2 micrograms/ml clindamycin into standard blood agar. Using this medium, the time and materials required to identify and isolate Pasteurella multocida was substantially reduced with only a slight loss in sensitivity. The combination of 2 micrograms/ml clindamycin and 4 micrograms/ml neomycin was effective in isolating Bordetella bronchiseptica from rabbits.

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What this paper is about

Easily prepared and inexpensive culture medium for the isolation of Pasteurella multocida and Bordetella bronchiseptica from the nares of laboratory rabbits was prepared by incorporating 2 micrograms/ml clindamycin into standard blood agar. Using this medium, the time and materials required to identify and isolate Pasteurella multocida was substantially reduced with only a slight loss in sensitivity. The combination of 2 micrograms/ml clindamycin and 4 micrograms/ml neomycin was effective in isolating Bordetella bronchiseptica from rabbits.

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Available abstract

Easily prepared and inexpensive culture medium for the isolation of Pasteurella multocida and Bordetella bronchiseptica from the nares of laboratory rabbits was prepared by incorporating 2 micrograms/ml clindamycin into standard blood agar. Using this medium, the time and materials required to identify and isolate Pasteurella multocida was substantially reduced with only a slight loss in sensitivity. The combination of 2 micrograms/ml clindamycin and 4 micrograms/ml neomycin was effective in isolating Bordetella bronchiseptica from rabbits.

Key concepts: Bordetella bronchiseptica, Pasteurella multocida, Microbiology, Clindamycin, Bordetella, Pasteurella, Agar, Neomycin

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Selective media for Pasteurella multocida and Bordetella bronchiseptica. — Research Paper | ScholarLens