Amplification of Chlamydia trachomatis in Animal Cell Host

Abundant amount of Chiamydia trachomatis could be amplified in mammalian McCoy cells and purified using descontinuous Uroarafin gradient centrifugation. As a chemical means io increase the Chlamydia trachomatis inclusions in McCoy cells IUdR treatment was found to be more effective than the cycloheximide treatment and was recommendanble for the proliferation of Chlamydia trachomatis. Centrifugation promoted Chlamydia trachomatis adhesion to McCoy cell surface, and maximal percentage of infected cells was obtained at about 3000g. The purified Chlamydia trachomatis could be kept in SPG solution for 48 hours at +4 but for longer storage freezing to -7 was necessary.