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[Construction of Chinese genomic cosmid library].

Xin Qin, Jian Zhang, Jinhwa Kong, Yanting Shen, Guoliang Wu

Abstract

A Chinese genomic library has been constructed using SuperCos1 cosmid vector. 6.09 x 10(5) clones were obtained with an everage insert size of 40 kb ranging from 32 to 45 kb, which cover approximately 8.12 fold human genomic DNA. DNA pools prepared from the total library were screened with 6 known markers distributed on different chromosomes, which all were tested positive.

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Available abstract

Key concepts: Cosmid, genomic DNA, Insert (composites), Genomic library, Biology, Genetics, DNA, Molecular biology