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1991•Journal of Microbiology and BiotechnologyRequires access

Molecular Cloning of $\beta$-Galactosidase from Bacillus subtilis HP-4

Jeong‐Ho Kim, Jae-Chang Lee, Jeong-Won Huh, Ki‐Chul Chung

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Abstract

A gene coding for a -galactosidase of Bacillus subtilis HP-4 was cloned in E. coli JM109 by inserting HindIII digested fragment of B. subtilis HP-4 chromosomal DNA into the site of pBR322 and selecting recombinant transformant showing blue color on X-gal plate. The recombinant plasmid, named pBG109, was found to contain the 1.4 Kbp HindIII fragment originated from B. subtilis HP-4 chromosomal DNA by Southern hybridization. The cloned gene was stably maintained and expressed in E. coli JM109 and the pBG109 encoded -galactosidase had the same enzymatic properties as those of -galactosidase produced by B. subtilis HP-4.

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What this paper is about

A gene coding for a -galactosidase of Bacillus subtilis HP-4 was cloned in E. coli JM109 by inserting HindIII digested fragment of B. subtilis HP-4 chromosomal DNA into the site of pBR322 and selecting recombinant transformant showing blue color on X-gal plate. The recombinant plasmid, named pBG109, was found to contain the 1.4 Kbp HindIII fragment originated from B. subtilis HP-4 chromosomal DNA by Southern hybridization. The cloned gene was stably maintained and expressed in E. coli JM109 and the pBG109 encoded -galactosidase had the same enzymatic properties as those of -galactosidase produced by B. subtilis HP-4.

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Available abstract

A gene coding for a -galactosidase of Bacillus subtilis HP-4 was cloned in E. coli JM109 by inserting HindIII digested fragment of B. subtilis HP-4 chromosomal DNA into the site of pBR322 and selecting recombinant transformant showing blue color on X-gal plate. The recombinant plasmid, named pBG109, was found to contain the 1.4 Kbp HindIII fragment originated from B. subtilis HP-4 chromosomal DNA by Southern hybridization. The cloned gene was stably maintained and expressed in E. coli JM109 and the pBG109 encoded -galactosidase had the same enzymatic properties as those of -galactosidase produced by B. subtilis HP-4.

Key concepts: Bacillus subtilis, PBR322, Plasmid, Recombinant DNA, Molecular biology, Molecular cloning, Biology, HindIII

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