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[Assay of 2-phenylbutyric acid in plasma with gas and liquid chromatography].

Tsamis Mt, Mange Am, Robert Farinotti, G. Mahuzier

Abstract

Two chromatographic methods which allow the measurement of 2-phenylbutyric acid in serum are described: a gas chromatographic, after silylation, and a reversed-phase high-performance liquid chromatographic. The liquid chromatography with a fluorescent detection, after derivatization by 4-bromomethyl-7-methoxycoumarin, is ten times more sensitive than gas chromatography and 50 ng/ml can be measured in biological liquids.

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Available abstract

Key concepts: Chemistry, Chromatography, Derivatization, Silylation, Gas chromatography, High-performance liquid chromatography, Organic chemistry, Catalysis