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2009•Journal of the Society of Cosmetic Scientists of KoreaRequires access

In Vitro Alternative Phototoxicity Test for Various Cosmetic Natural Extracts and Chemicals

Wan‐Goo Cho, Jee-Eun Park, Mun-Euek Park, Sangmin Lee

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Abstract

This study was done to assess an alternative method as a replacement of in vivo phototoxicity test. The human fibroblasts were exposed to several phototoxic chemicals (promethazine, chlorpromazine chlortetracycline, 8-methoxypsoralen, neutral red, bithionol) and non-phototoxic materials (cinnamic aldehyde, p-aminobenzoic acid, sodium lauryl sulfate, L-cysteine). The cell viability was measured by neutral red uptake (NRU) assay. The results of the NRU phototoxicity (PT) assay showed a close agreement with in vivo test except bithionol. We also have tested the cosmetic ingredients including A, D, 106W, EL-1, Carex humilis L. extract, Canna indica L. extract, Salvia miltiorrhira Bunge extract, MCX and 1789. Most materials except Salvia miltiorrhira Bunge extract did not show any phototoxicity.

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What this paper is about

This study was done to assess an alternative method as a replacement of in vivo phototoxicity test. The human fibroblasts were exposed to several phototoxic chemicals (promethazine, chlorpromazine chlortetracycline, 8-methoxypsoralen, neutral red, bithionol) and non-phototoxic materials (cinnamic aldehyde, p-aminobenzoic acid, sodium lauryl sulfate, L-cysteine). The cell viability was measured by neutral red uptake (NRU) assay. The results of the NRU phototoxicity (PT) assay showed a close agreement with in vivo test except bithionol. We also have tested the cosmetic ingredients including A, D, 106W, EL-1, Carex humilis L. extract, Canna indica L. extract, Salvia miltiorrhira Bunge extract, MCX and 1789. Most materials except Salvia miltiorrhira Bunge extract did not show any phototoxicity.

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Available abstract

This study was done to assess an alternative method as a replacement of in vivo phototoxicity test. The human fibroblasts were exposed to several phototoxic chemicals (promethazine, chlorpromazine chlortetracycline, 8-methoxypsoralen, neutral red, bithionol) and non-phototoxic materials (cinnamic aldehyde, p-aminobenzoic acid, sodium lauryl sulfate, L-cysteine). The cell viability was measured by neutral red uptake (NRU) assay. The results of the NRU phototoxicity (PT) assay showed a close agreement with in vivo test except bithionol. We also have tested the cosmetic ingredients including A, D, 106W, EL-1, Carex humilis L. extract, Canna indica L. extract, Salvia miltiorrhira Bunge extract, MCX and 1789. Most materials except Salvia miltiorrhira Bunge extract did not show any phototoxicity.

Key concepts: Phototoxicity, Chemistry, Pharmacology, Viability assay, Neutral red, In vivo, Chelidonium, Traditional medicine

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