Estimation of concentration of spermatozoa in human semen by determination of DNA.

Sperm concentration of 265 semen samples were coloimetrically estimated using carefully counted samples of semen or DNA solution as standards. This method is an alternative to the hemocytometer method usually used and it should yield increased accuracy. The majority of males had high sperm counts and normal sperm morphology. The range of clinical interest 1 million to 1 billion sperm/ml is covered by a 5- to 50-fold dilution range. Values observed in this study appear to be more accurate than those provided by hemocytometer counts.