1994•PubMedRequires access

An improved fluor diffusion assay for chloramphenicol acetyltransferase gene expression.

Purschke Wg, Müller Pk

Abstract

We report on a substantial improvement of the widely used fluor diffusion assay for chloramphenicol acetyltransferase (CAT) activity. The stable and inexpensive [3H]NaAcetate along with yeast acetyl CoA synthetase is used to produce [3H]acetyl CoA with high specific radioactivity and high yield. In a second step, the enzymatically produced [3H]acetyl CoA is introduced as a substrate for CAT in the fluor diffusion assay. Due to these modifications, the assay becomes more sensitive, the range of linearity is increased by two orders of magnitude and the assay becomes less costly.

About this research paper

What this paper is about

Why it matters

OpenAlex reports 14 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Chloramphenicol acetyltransferase, Acetyltransferase, Chloramphenicol, Molecular biology, Chemistry, Yeast, Substrate (aquarium), Acetyltransferases