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[Fractionation of urinary glycosaminoglycans using ethanol, their purification and study].

Kosiagin Dv

Abstract

A new method for the isolation of the urine glycosaminoglycans (GAG) with the use of ethanol is suggested. Experimental conditions for ethanol sedimentation and purification of GAG have been selected. It is suggested that the urine GAGs be divided into sulfated and nonsulfated with the use of resochin and their uronic acids and hexoses be examined. The normal GAG level and composition in humans have been determined.

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Available abstract

Key concepts: Glycosaminoglycan, Uronic acid, Chemistry, Fractionation, Urine, Chromatography, Ethanol, Sulfation