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Effect of bacterial association on virulence of Entamoeba histolytica to baby hamster kidney cell monolayers.

Prachetash Ghosh, Sandeep Gupta, S. Naik, A Ayyagarí, S. R. Naik

Abstract

Axenic E. histolytica trophozoite strain NIH:200 and HMI:IMSS when co-associated with aerobic bacteria Escherichia coli strain K12 and serotype 056 showed marked increase in virulence as observed by destruction of baby hamster kidney (BHK) monolayers. However, when incubated with anaerobic bacterial strains Clostridium perfringens and Bacteroides fragilis virulence remained unaltered. Further, adherence of E. histolytica to BHK monolayer was found to be mediated by N-acetyl-D-galactosamine.

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Key concepts: Virulence, Entamoeba histolytica, Microbiology, Clostridium perfringens, Axenic, Baby hamster kidney cell, Hamster, Biology