[Leukemic immunophenotyping with flow cytometry].

We have evaluated the clinical efficacy and problematic items of flow cytometric surface and intracellular immunophenotyping of leukemic cells. The multi-parametric analysis on the results of leukemic phenotyping displayed a possibility of an independent phenotypical classification, but concurrently we recognized complementary relationships between the morphological and phenotypic approaches. Defective information on the morphology of leukemic cells unexpectedly introduced an insufficient quality in performance of leukemic phenotyping. On the other hand, the interpretations of phenotypic outcomes were complicated in cases with mixed leukemia and partial expression of a marker. Finally we hope further integration of the expression spectrum of markers, including the improvement of CD nomenclature system.