2002•PubMedRequires access
[Construction and expression of a vector containing protein transduction domain and bcr/abl fusion gene].
Yingmin Liang, Qiang Sun, Shanshan Jiang, Jizhu Wang, Rong-Li Wu, Ping Chen, Li Liu, Hua Han
Open publisher page 2 citations
Abstract
OBJECTIVE: To construct a vector containing protein transduction domain (PTD) and bcr/abl fusion gene of chronic myelogenous leukemia and express PTD-bcr/abl fusion protein in E. Coli. METHODS: DNA fragment encoding PTD was synthesized and fused to PCR-amplified bcr/abl gene fragment, then inserted into plasmid pET-16b to get the expression vector pEPb containing PTD-bcr/abl fusion gene, which was transfected and expressed in E. Coli LB21. PTD-bcr/abl fusion protein was purified by affinity chromatography. RESULTS: 523 bp bcr/abl fusion gene was effectively amplified. The PTD-bcr/abl gene sequencing showed the same sequence as scheduled. The fusion peptide was successfully expressed in E. Coli and purified. CONCLUSION: The results may provide a new PTD-bcr/abl fusion peptide for the immunotherapy of CML.