[New type II and IIs restriction endonucleases from thermophilic bacteria of the genus Bacillus].
Repin Ve, Serov Gd, Puchkova Li, Tereshchenko Ta, Lebedev Lr, Chizhikov Ve
Abstract
Repin Ve, Serov Gd, Puchkova Li, Tereshchenko Ta, Lebedev Lr, Chizhikov Ve
Abstract
Restriction endonucleases have been isolated from 26 strains of thermophilic strains of the Bacillus genus, their recognition sequences were determined, and for 15 of them cleavage sites identified. The enzymes proved to be isoschizomers of known endonucleases BstNI, EarI, HaeIII, HpaII, Cfr10I, BsiYI, BclI, BbvII, BbvI, BstEII, BsaBI, BsrI, FspI, ClaI, SfeI.
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Restriction endonucleases have been isolated from 26 strains of thermophilic strains of the Bacillus genus, their recognition sequences were determined, and for 15 of them cleavage sites identified. The enzymes proved to be isoschizomers of known endonucleases BstNI, EarI, HaeIII, HpaII, Cfr10I, BsiYI, BclI, BbvII, BbvI, BstEII, BsaBI, BsrI, FspI, ClaI, SfeI.
Key concepts: Isoschizomer, HaeIII, Restriction enzyme, HpaII, Chemistry, Thermophile, Bacillus (shape), Genus