Low Temperature Preservation of Mouse Lymphocytes with Dimethyl Sulfoxide

Abstract Mouse lymphocytes have been successfully preserved for 3 months at -196 C. in the presence of 15 per cent dimethyl sulfoxide. Other concentrations of dimethyl sulfoxide or glycerol were less effective. The viability of the frozen lymphocytes was assessed by phase contrast microscopy, protein synthesizing activity and by the ability of these lymphocytes to produce splenomegaly when injected with F1 hybrids.