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[Detection of TEM-beta-lactamase in strains of Haemophilus influenzae resistant to ampicillin using the polymerase chain reaction (PCR)].

Espinosa de los Monteros Le, Cíntia Renata Costa Rocha, Catalina Gayosso, Junji Tanaka, A Pérez-Miravete, Santos Ji

Abstract

With the purpose of determining the type of beta-lactamases that mediate ampicillin resistance to in Haemophilus influenzae strains in the Hospital Infantil de México "Federico Gómez", we determined the minimum inhibitory concentration of 180 strains, isolated from different sources, to ampicillin, amoxicillin-clavulanic acid and ampicillin-sulbactam. All ampicillin resistant strains (29) were beta-lactamase positive as determined by nitrocephin hydrolysis. Using PCR with the primers from pBR322, we detected the presence of the gene for the TEM-beta-lactamase in 100% of the ampicillin resistant strains.

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Key concepts: Ampicillin, Amp resistance, Clavulanic acid, Haemophilus influenzae, Microbiology, Beta-lactamase, Sulbactam, Polymerase chain reaction