2014•PubMedRequires access
[Adrenomedullin inhibits TGF-β1-induced procollagen expression in cultured human fetal lung fibroblasts via Smad2/3 pathway].
Shuling Hao, Weiping Wang, YU Zhong-he, Jiandong Li
Open publisher page 1 citations
Abstract
OBJECTIVE: To observe the effects of adrenomedullin (AM) on transforming growth factor- β1 (TGF-β1) induced the expression of procollagen type 1 alpha 1 (Col1α1) and procollagen type 3 alpha 1 (Col3α1) as well as Smad2/3 phosphorylation in human fetal lung fibroblasts (HFLFs). METHODS: Primary HFLFs were cultured in vitro. After treated with TGF-β1 and/or AM, the expression levels of Col1α1 and Col3α1 mRNA were determined by reverse transcription PCR (RT-PCR), and phospho-Smad2/3 (p-Smad2/3) protein levels in HFLFs were measured by Western blotting. RESULTS: TGF-β1 increased the gene expression levels of Col1α1 and Col3α1, and promoted the phosphorylation levels of Smad2/3 protein in HFLFs. AM significantly reversed the expression level of Col3α1 mRNA, and inhibited p-Smad2/3 expression in HFLFs induced by TGF-β1. CONCLUSION: AM could inhibit TGF-β1-induced the procollagen expression in cultured HFLFs possibly through the Smad2/3 signaling pathway.