2010•Methods in molecular biologyRequires access

Adipogenic Differentiation of Adult Equine Mesenchymal Stromal Cells

Martin A. Vidal, Mandi J. Lopez

Abstract

Equine adipose tissue-derived mesenchymal stem cells (ASCs) have only recently been investigated for their adipogenic, chondrogenic, and osteogenic differentiation potential. This chapter will briefly outline the molecular mechanisms leading to adipogenesis and the methods of equine adipose tissue harvest, ASC isolation, and adipogenic differentiation. The reader is also directed to other reported methods of adipogenesis for ASCs and mesenchymal stem cells (MSCs) from other tissues.

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Key concepts: Adipogenesis, Mesenchymal stem cell, Adipose tissue, Chondrogenesis, Cell biology, Stromal cell, Stem cell, Biology