[Expression of heat shock proteins in laryngeal carcinoma].

OBJECTIVE: To determine the expression of HSPs(HSP90 alpha, HSP90 beta, HSP70, HSP27) in laryngeal carcinoma and normal laryngeal mucosa. METHODS: Total RNA were extracted from 21 laryngeal cancer tissue samples and the normal mucosa of the corresponding larynx. The expression of HSPs mRNA was examined by Slot blot analysis. RESULTS: 1. HSP90 alpha and HSP70 were selectively overexpressed 5 times more in laryngeal carcinoma than in normal mucosa of larynx. 2. HSP90 beta and HSP27 were constitutively expressed at low level in these two groups. There was no significant difference between the levels of expression of HSP90 beta and HSP27 in laryngeal carcinoma and normal controls. CONCLUSION: The results that HSP90 alpha and HSP 70 mRNA were selectively overexpressed in laryngeal carcinoma suggested that HSP90 alpha and HSP70 might play a specific role in the pathogenesis of laryngeal cancer. To clarify the mechanism of HSPs action could help to provide theorical basis for the biological therapy of laryngeal cancer.