1995•PubMedRequires access

[Electrophoretic analysis on the proteolytic decomposition of of FSH preparations].

Hosni Khalaf, Ulf P. Neumann, Matthäus M. RIMPLER

Abstract

We investigated the influence of some proteolytic enzymes being present in the digestive canal (pepsin, trypsin, chymotrypsin) on the digestion of porcine FSH-samples by electrophoresis. Trypsin and Chymotrypsin digested the 18-kDa-protein much faster than pepsin, whereas a 67-kDa-protein was much faster digested by Pepsin than by Trypsin or Chymotrypsin. The overall proteolytic effect of Chymotrypsin is small compared with Pepsin and Trypsin. A subsequent digestion with Pepsin, Trypsin and Chymotrypsin led to a complete proteolysis of all proteins being present in the sample.

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Key concepts: Trypsin, Pepsin, Chymotrypsin, Proteolysis, Chemistry, Biochemistry, Proteolytic enzymes, Digestion (alchemy)