2005•PubMedRequires access

[The assay of tissue collagenase activity using fluorescein isothiocyanate labeled collagen].

Ryzhakova Os, N I Solov'eva

Abstract

This report describes the assay of collagenolytic activity, particularly activity of tissue collagenases, using the reconstituted fibrils of fluorescein-labeled collagen type I as substrate. Labeling of soluble rat skin collagen was carried out by the modified method of Baichi et al 1980. This method is very sensitive, reproducible and useful for screening large number of samples.

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Key concepts: Collagenase, Fluorescein, Fluorescein isothiocyanate, Chemistry, Substrate (aquarium), Chromatography, Biochemistry, Molecular biology