[Construction and identification of plasmid vector encoding two survivin shRNA].

OBJECTIVE: To construct and identify the eukaryotic expression plasmids encoding two short hairpin RNA (shRNA) of survivin for the purpose of paving the way for the studies of targeted gene therapy for prostatic carcinoma (PCa). METHODS: Two shRNA of survivin were designed and synthesized respectively, and then both were cloned into plasmids. Finally, the recombinant plasmids were confirmed by sequencing and agarose gel electrophoresis after restriction digestion. RESULTS: The recombinant plasmids encoding two survivin shRNA were constructed and the aim sequence obtained. CONCLUSION: Successful construction of the recombinant provides a sound basis for the research of targeted gene therapy for PCa.