[Establishment of a drug resistant leukemia cell line caused by mdr1 gene].

OBJECTIVE: To establish a drug resistant leukemia cell line caused by mdr1 gene for the mdr1 reversing research. METHODS: The leukemia sensitive cell line K562 was transfected with mdr1 cDNA by liposome-induced gene transfection and selected by G418. The mdr1 phenotype was identified by RT-PCR, the P-gp expression was detected by flow cytometry, the function of P-gp was measured by Rh123 efflux experiment, and the sensitivity of cell lines to the drug was detected by MTT test. Results The transfected K562/mdr1 with mdr1 cDNA highly expressed mdr1 and showed a remarkable drug resistant feature. CONCLUSION: mdr1 plays an important role in drug resistance and the mdr1 cell line established by gene transfection can provide a better cell model for mdr1 reversing research.