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[Restriction mapping of plasmid pSa1, isolated from Streptomyces albus G strain].

Orekhov Av, Rebentish Ba, Lomovskaia Nd

Abstract

A novel plasmid designated pSa1 has been isolated from Streptomyces albus G strain producing SalGI restriction endonuclease. Molecular weight of the plasmid is 3.4 +/- 0.2 mD. The action of 12 restriction endonucleases on the plasmid DNA was studied. Restriction map of pSa1 DNA was established for SmaI, HindII, XbaI and KpnI endonucleases.

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Available abstract

Key concepts: Restriction enzyme, Plasmid, Biology, Restriction map, Streptomyces albus, Strain (injury), Genetics, Restriction site