Flow Cytometry Detection of CD44s and CD44v6 in Primary Gastric Carcinoma

Purpose To explore the effect of adhesion molecule CD44s and CD44v6 expressions in gastric carcinoma. Methods In 58 human gastric carcinoma and 40 benign gastric mucosa disease,the expressions of CD44s and CD44v6 in were examined by flow cytometry and PCNA LI was determined by immunohistochemical staining. Results The expression of CD44s was significantly higher in gastric carcinoma (62.1%,36/58) than that in benign gastric mucosa disease (27.5%,11/40, P 0.01) and was associated with PCNA LI ( P 0.05).The expression of CD44v6 was significantly higher in gastric carcinoma (58.6%,34/58) than that in benign gastric mucosa disease (32.5%,13/40, P 0.05) and was related to tumor localization ( P 0.05 ) lymph node metastasis ( P 0.05) and PCNA LI ( P 0.05).A good correlation was observed between the expressions of CD44s and CD44v6 in benign gastric mucosa disease ( R s=0.59,P0.01) and in gastric carcinoma (R s=0.29,P0.05) . Conclusions In gastric carcinoma,CD44s and CD44v6 are overexpressed and correlated with cell proliferative activity,and CD44v6 is also related to tumor localization and lymph node metastasis.A good correlation exists between the expressions of CD44s and CD44v6