Effects of Aspisol on Proliferation and Apoptosis of Hela Cells

Objective To explore the effects of aspisol on hela cells' proliferation,apoptosis and cell cycle.Methods Hela cells were randomly divided into three study groups(aspisol with 1,5,10 mmol/L) and one control group.The level of cell proliferation treated with aspisol was assessed by drawing the proliferation curves of HeLa cells.Hela cells were incubated with aspisol at various concentrations for 24 h,the morphological changes of Hela cells were evaluated by HE staining and AO/EB staining,apoptosis rate in Hela cells was analyzed by Flow Cytometry(FCM).The cell cycle of Hela cells by aspisol for 24h was analyzed by flow cytometry.Results The results of the proliferation curves demonstrated that compared with the control group,treatment with aspisol at 1 to 10 mmol/L for 1-6 days significantly inhibited growth of Hela cells in time and dose dependent manners,HE staining shows that under light microscope the cells in aspisol treatment groups showed decreased density,the cells became smaller,round shape and light staining of nucleus.Compared with the control group,the early apoptotic rates were(5.73±0.87)%,(19.11±2.86)%,(33.72±5.06)% after the exposure of the cells to aspisol at 1,5 and 10 mmol/L for 24 h respectively.Further analysis of cell cycle by flow cytometry indicated that effects of aspisol on Hela cells for 24 h led to G0/G1phase arrest.Conclusion Aspisol can inhibit cervical cancer Hela cells proliferation、induce apoptosis and prevent cell cycle progression.