Expression changes of tumor necrosis factor mRNA and protein in PBMC of patients with chronic heart failure and influence of ACEI on it

Objective To study the expression changes of tumor necrosis factor(TNF-α) mRNA and protein in patients with chronic heart failure and influence of angiotensin converting enzyme inhibitors(ACEI) on it. Methods Peripheral blood mononuclear cells (PBMC) was isolated from plasma, which was cultured with RPMI1640 medium for 24h,the conclusion of ACEI was respectively 0 mmol/L, 10 -8 mmol/L,then levels of TNF-α mRNA and protein of PBMC was determined by RT-PCR and Western blot in 20 patients with CHF and 10 health controls. Results The level of TNF-α mRNA and protein of PBMC of patients were higher than the controls. Capoten can inhibit the expression of TNF-α. Conclusion The expression of tumor necrosis factor in patients with chronic heart failure was up regulated, and ACEI can inhibit the expression of TNF-α,which suggested TNF-α and RAS interacted in the development of CHF and PBMC may be the one of source of TNF-α.