Effects and mechanisms of silencing caveolin-1 gene by siRNA on cell migration and invasion in pancreatic carcinoma cells

OBJECTIVE: To investigate the effect of sliencing caveolin-1 gene by siRNA on cell migration and invasion in human pancreatic carcinoma PANC1 and elucidate the mechanism.METHODS: The plasmid pBCMGneo-Sequence-2 and its corresponding empty vector(pBCMGneo-shuffle-2) were transfected into PANC1 cell line.caveolin-1 expression in transfectants was subsequently confirmed by western blot analysis.A pair of monoclonal cell lines,PANC1/siRNA and PANC1/shuffle were choosed for further studies.Anoikis assay was tested and analysed by flow cytometry(FCM).Cell adhesive ability was detected by cell adhesive assay.Migration and invasion capability of tumor cells were also tested by chamber invasion assay.MMP-7 were determined by western blot analysis in both cell groups.RESULTS: caveolin-1 was down-regulated in PANC1/siRNA as compared with PANC1/shuffle and PANC1(P0.01).Apoptosis index of PANC1/siRNA was lower than that of PANC1/shuffle((9.24±1.61)% vs(17.32±1.79)%,P0.01).The knockdown of caveolin-1 in PANC1 cells enhanced adhesive capability(0.867±0.153 vs 0.523±0.072,P0.01).Additionally,the knockdown of caveolin-1 enhanced cells to migrate(142.6±24.35 vs 106.5±10.81,P0.05).The invasive capability was enhanced obviously(88.9±8.07 vs 61.3±7.23,P0.01).It also demonstrated that MMP-7 protein was increased in PANC1/shuffle(P0.01).CONCLUSION: The results provide an initial demonstration that caveolin-1 can inhibit the migration and invasion through regulating the expression of MMP-7 protein in PANC1 cells.