2009•Neural Injury and Functional ReconstructionRequires access
Construction and Identification of Retroviral Vector Expressing TH Gene
Zhu Sui-qian
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Abstract
Objective:To construct and identify the recombinant retrovirus vector encoding tyrosine hydroxylase(TH) gene,establish the stable cell line after transfection.Methods:TH gene was cloned into the retroviral vector pLNCX2-MCS.High titer viral particles of pLNCX2-TH were obtained after transfection of PT67 cell by Lipofectine 2000.Then the recombinant retrovirus was transfected into NIH3T3 cells and the titer was measured.Results:Restriction,sequencing analysis confirmed that the human TH cDNA was successfully inserted into the retroviral vector.The titer of recombinant retrovirus with TH gene was 1.6×106 pfu/mL and the retrovirus was effectively transfected into NIH3T3 cells.Conclusion:The recombinant retrovirus containing TH gene is successfully constructed.